Topical Mupirocin Treatment Reduces Interferon and Myeloid Signatures in Cutaneous Lupus Erythematous Lesions Through Targeting of Staphylococcus Species.

Type I interferons (IFNs) promote inflammatory responses and are elevated in CLE lesions.

I型干扰素（IFNs）促进炎症反应，并在CLE病变中升高。

We recently reported that CLE lesions are frequently colonized with Staphylococcus aureus.

我们最近报告称，CLE病变部位常有金黄色葡萄球菌定植。

Here, we follow up via a proof-of-concept study to investigate whether type I IFN and inflammatory gene signatures in CLE lesions can be modulated with mupirocin, a topical antibiotic treatment against S aureus-mediated skin infections.

在这里，我们通过一个概念验证研究来跟进，调查在金黄色葡萄球菌介导的皮肤感染中，CLE病变部位的一型干扰素和炎症基因表达谱是否可以通过局部抗生素治疗药物莫匹罗星进行调节。

Methods

Participants with active CLE lesions (n = 12) were recruited and randomized into a week of topical treatment with either 2% mupirocin or petroleum jelly vehicle.

招募了12名患有活动性CLE病变的参与者，并将他们随机分为一组，进行为期一周的局部治疗，治疗方案为2%莫匹罗星或凡士林基质对照。

Paired samples were collected before and after seven days of treatment to assess microbial lesional skin responses.

在治疗前和治疗七天后收集配对样本，以评估微生物病变皮肤的反应。

Microbial samples from nares and lesional skin were used to determine baseline and posttreatment Staphylococcus abundance and microbial community profiles by 16S ribosomal RNA gene sequencing.

通过16S核糖体RNA基因测序，使用鼻孔和病变皮肤的微生物样本来确定基线和治疗后金黄色葡萄球菌的丰度以及微生物群落剖面。

Inflammatory responses were evaluated by bulk RNA sequencing of lesional skin biopsies.

通过病变皮肤活检样本的批量RNA测序评估炎症反应。

Results

We identified 173 differentially expressed genes in CLE lesions after topical mupirocin treatment.

我们在接受局部莫匹罗星治疗的CLE病变中鉴定了173个差异表达基因。

Decreased lesional Staphylococcus burden correlated with decreased IFN pathway signaling and inflammatory gene expression and barrier dysfunction.

病变部位的金黄色葡萄球菌负荷减少与IFN通路信号传导和炎症基因表达降低以及屏障功能障碍减少相关。

Interestingly, mupirocin treatment lowered skin monocyte levels, and this mupirocin-associated depletion of monocytes correlated with decreased inflammatory gene expression.

有趣的是，莫匹罗星治疗降低了皮肤单核细胞水平，这种莫匹罗星相关的单核细胞耗竭与炎症基因表达的减少相关。

Conclusions

Mupirocin treatment decreased lesional Staphylococcus, and this correlated with decreased IFN signaling and inflammatory gene expression.

莫匹罗星治疗减少了病灶处的葡萄球菌，这与干扰素信号传导和炎症基因表达的减少相关。

This study suggests a topical antibiotic could be employed to decrease lupus skin inflammation and type I IFN responses by reducing Staphylococcus colonization.

本研究建议，局部抗生素可以用来减少由金黄色葡萄球菌定植引起的红斑狼疮皮肤炎症和I型干扰素反应。