The Gut Microbiome at the Onset of Inflammatory Bowel Disease: A Systematic Review and Unified Bioinformatic Synthesis.

We present a novel secondary bioinformatic reanalysis of sequence outputs mapped to the latest microbial taxonomy.

我们提出了一种新的次要生物信息学再分析，将序列输出映射到最新的微生物分类学。

Methods

MEDLINE and Embase searches were performed for microbiome studies in treatment-naïve IBD.

对治疗未使用炎症性肠病(IBD)的微生物组研究进行了MEDLINE和Embase检索。

Appraisal was completed with Risk Of Bias In Non-randomized Studies - of Exposures (ROBINS-E).

评估工作已完成，使用的是非随机研究的偏倚风险评估工具 - 暴露因素(ROBINS-E)。

Available 16S ribosomal RNA sequence data sets were downloaded and missing data sets requested.

下载了可用的16S核糖体RNA序列数据集，并请求了缺失的数据集。

Integrated data were run through a unified QIIME2 bioinformatics pipeline.

整合后的数据通过统一的QIIME2生物信息学流程进行处理。

Multivariable models adjusting for methodologic differences were developed using MaAsLin2.

使用MaAsLin2开发了调整方法学差异的多变量模型。

Results

There were 36 eligible studies; 18 contributed to bioinformatic reanalysis and 24 to supplementary meta-analysis.

共有36项符合条件的研究；其中18项参与了生物信息学再分析，另外24项参与了补充的荟萃分析。

Samples from 1743 patients were included, comprising 678 from individuals with Crohn's disease (CD), 399 with ulcerative colitis (UC), 130 healthy controls (HCs), and 405 symptomatic controls (SCs); 990 of which were biopsy samples.

纳入了1743名患者的样本，包括678名克罗恩病（CD）患者、399名溃疡性结肠炎（UC）患者、130名健康对照（HCs）和405名有症状对照（SCs）；其中990个样本为活组织检查样本。

Alpha diversity was reduced: feces-pediatric UC vs SCs, adult CD and UC vs HCs, and pediatric SCs vs HCs; pediatric biopsy samples-CD vs SCs, CD vs UC, and UC vs SCs.

Alpha多样性降低：粪便样本中，儿童UC患者与SCs、成人CD和UC患者与HCs、儿童SCs与HCs相比；儿童活组织检查样本中，CD与SCs、CD与UC、UC与SCs相比。

Beta diversity demonstrated clear distinctions between fecal and mucosal biopsy communities, least evident in UC, in addition to community separation by geography.

Beta多样性在粪便和粘膜活检样本的微生物群落之间显示出明显的差异，这种差异在溃疡性结肠炎（UC）中最不明显，除此之外，还显示出按地理区域分隔的微生物群落。

Multivariate modeling revealed depletion of anaerobic and enrichment of aerobic and facultative anaerobic bacteria, alongside enrichment of oral genera across both CD and UC.

多变量建模揭示了厌氧菌的减少和需氧菌及兼性厌氧菌的富集，以及在克罗恩病（CD）和溃疡性结肠炎（UC）中口腔菌属的富集。

Conclusions

Core microbial perturbations at onset of CD and UC are depletion of anaerobes and enrichment of oxygen-tolerant, orally associated bacteria.

在克罗恩病（CD）和溃疡性结肠炎（UC）发病初期，微生物群的主要变化是厌氧菌的减少和耐氧、与口腔相关的细菌的富集。

As we place greater emphasis on early diagnosis and prediction of IBD risk, this finding may support innovative diagnostic approaches.

随着我们对炎症性肠病（IBD）早期诊断和风险预测的重视程度增加，这一发现可能支持创新的诊断方法。

Microbiome-targeted intervention and alteration of luminal oxygen availability may offer novel therapeutic avenues for new-onset patients and identified high-risk groups.

针对微生物群的干预和腔内氧可用性的改变可能为新发患者和已识别的高风险群体提供新的治疗途径。