Dengue Suppression by Male Wolbachia-Infected Mosquitoes.

Repeated releases of wolbachia-infected males can potentially suppress wild-type mosquito populations and reduce the risk of dengue virus infection.

反复释放感染了wolbachia的雄性蚊子有可能抑制野生型蚊子种群，并降低登革热病毒感染的风险。

Methods

We conducted a trial involving the release of male A. aegypti mosquitoes infected with the wAlbB strain of wolbachia bacteria for the control of dengue in Singapore, a tropical city-state.

我们在新加坡这个热带城市国家进行了一项试验，释放了感染wAlbB型沃尔巴克氏菌的雄性埃及伊蚊，以控制登革热。

In this cluster-randomized trial with test-negative controls, we divided 15 geographic population clusters into two groups: 8 clusters received deployments of male wolbachia-infected mosquitoes (intervention clusters) and 7 clusters received no deployments (control clusters).

在这项以测试阴性对照的群组随机试验中，我们将15个地理人口群组分为两组：8个群组接受了雄性沃尔巴克氏菌感染蚊子的部署（干预群组），而7个群组没有接受部署（对照群组）。

The primary end point was the diagnosis of symptomatic dengue virus infection of any severity caused by any serotype of the virus, as measured by the odds ratio for the distribution of wolbachia exposure among laboratory-confirmed reported dengue cases as compared with test-negative controls.

主要终点是诊断出由任何血清型病毒引起的任何严重程度的登革热病毒感染症状，通过实验室确诊的报告病例中沃尔巴赫氏体暴露分布的比值比与测试阴性对照组进行比较测量。

Results

A total of 393,236 residents lived in the intervention clusters, and 331,192 lived in the control clusters.

共有393,236名居民生活在干预群组中，而331,192名居民生活在对照群组中。

Adult wild-type A. aegypti populations were suppressed across the intervention clusters.

在干预组群中，成年野生型埃及伊蚊种群得到了抑制。

The baseline average abundance of the mosquitoes (number of adult female mosquitoes trapped divided by number of traps) was 0.18 and 0.19 in the intervention and control clusters, respectively; from 3 months after the initiation of the intervention until the end of the 24-month trial period, the average abundance was 0.041 and 0.277, respectively.

基线时，干预组和对照组的蚊子平均丰度（捕获的成年雌蚊数量除以陷阱数量）分别为0.18和0.19；从干预开始后的3个月直到24个月试验期结束，平均丰度分别为0.041和0.277。

In the intention-to-treatanalysis at 6 months or more, the percentage of residents in the intervention clusters who were dengue-positive was lower than that in the control clusters (354 of 5722 tests [6%] vs. 1519 of 7080 tests [21%]).

在6个月或以上的意向治疗分析中，干预组居民的登革热阳性率低于对照组（5722次测试中有354次阳性[6%]对比7080次测试中有1519次阳性[21%]）

The protective efficacy of the intervention, calculated as (1 - odds ratio) × 100, ranged from 71 to 72% with 3 to 12 months or more of wolbachia mosquito exposure, as represented by odds ratios of 0.28 to 0.29.

干预措施的保护效果，计算方式为(1 - 比值比) × 100，随着3个月或12个月或更长时间的沃尔巴赫氏蚊子暴露，其范围在71%到72%之间，比值比为0.28到0.29

Conclusions

Release of sterile wolbachia-infected male A. aegypti mosquitoes reduced vector populations and the risk of dengue infection in Singapore.

在新加坡释放无菌沃尔巴克氏体感染的雄性埃及伊蚊，降低了媒介种群数量和登革热感染的风险。

(Funded by the Singapore Ministry of Finance and others; ClinicalTrials.gov number, NCT05505682.).

(由新加坡财政部和其他机构资助；ClinicalTrials.gov编号，NCT05505682。)